The Churches of Asia salute you - i.e. The Churches in Asia Minor. Ephesus was in this Asia, and it is clear from this that the apostle was not at Philippi; had he been at Philippi, as the subscription states, he would have said, The Churches of Macedonia, not the Churches of Asia, salute you. How these places lay, in reference to each other, the reader will at once perceive by consulting the map in Acts.
With the Church that is in their house - That is, the company of believers who generally worshipped there. There were no churches or chapels at that time built; and the assemblies of Christians were necessarily held in private houses. It appears that Aquila and Priscilla devoted their house to this purpose. The house of Philemon was of the same kind; Philemon 1:2. So was likewise the house of Nymphas, Colossians 4:15. See the note on Romans 16:5.
The churches of Asia - The word “Asia” in the New Testament usually denotes Asia Minor in general; see the note on Acts 2:9. It was sometimes used in a more limited sense, to denote the region around Ephesus, and of which Ephesus was the center and capital; see note, Acts 16:6. This is the region undoubtedly which is intended here.
Salute you - Greet you; send respectful and affectionate Christian regards; see the note at Romans 16:3.
Aquila and Priscilla - See the note on Acts 18:26.
Much in the Lord - With affectionate Christian salutations; or as Christians. Wishing the blessing and favor of the Lord.
With the church that is in their house - See the note at Romans 16:5.